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Autoradiograms out of steroid metabolites regarding vitellogenic ovarian hair follicles of your bambooleaf wrasse incubated having [7- 3 H]pregnenolone

Autoradiograms out of steroid metabolites regarding vitellogenic ovarian hair follicles of your bambooleaf wrasse incubated having [7- 3 H]pregnenolone

Seven metabolites was separated by narrow layer chromatography and you may put up with benzene:acetone (4:step 1). step one, E1; dos, AD; step 3, P5; cuatro, DHEA 17-P; 5, E2; 6, 1seven-P5; eight, 17,20?-P 17,21-P; and you will 8, 17,20?-P5. Pick Dining table step 1 for steroid abbreviations.

Table step three

To find the steroidogenic pathway from E2 synthesis, four almost every other radiolabeled steroid drugs were used just like the precursors to have in vitro incubation regarding follicles. Ones four precursors, E2 was synthesized out of [ fourteen C]DHEA, [ fourteen C]Advertising, and you can [ step 3 H]E1. Whenever follicles was incubated which have [ ldssingles işe yarıyor mu 3 H]17-P, E2 was not identified due to the fact metabolite, and some Offer is delivered. In most incubations, T development cannot feel thought, and [ 14 C]T was not entirely transformed into E2.

Dimension from solution steroid levels

Serum amounts of E2 and you can E1 exhibited similar profiles inside an excellent day’s spawning seasons (Fig. dos). In the hours, if developmental phase of one’s largest oocytes is LMN, solution E2 and you can E1 profile were somewhat high (E2= ng/ml, E1= 5.08 ng/ml). But by hour, when the stage of your own largest oocytes is Meters, the levels fell (E2=5.03 ng/ml, Estep 1=1.fifty ng/ml) and you will remained constant account within and hours, in the event that degrees of one’s biggest oocytes was indeed TY and you may EMN, respectively (E2=6.00 ?six.27 ng/ml, Estep 1=step 1.15–step one.79 ng/ml). However, T and you can Offer profile were constant (T=step 1.22–step 1.71 ng/ml, AD=1.23–step one.43 ng/ml) throughout the day and you will don’t differ significantly one of ovarian stages (Fig. step three).

Fig. 2

Changes in the serum levels of estradiol-17? (E2) and estrone (E1) in female bambooleaf wrasse sampled at different times of a day during the spawning season.